Determination of Tetracyclines in Milk and Dairy Products

Method:	SPE
Matrix:	Milk
Application No.:	101743
Columns:	ProElut PLS 150 mg / 6 mL 30/pk
Cat No.:	68004
Sample Pretreatment:	1. Sample preparation Dilute milk sample (20 mL) or 2 g solid dairy product with 20 mL H₂O, add 20 mL McIlvaine buffer, vortex for 2 min, centrifuge at 4,000 rpm for 10 min, take 20 mL supernatant as the sample solution. McIlvaine buffer: Disodium hydrogen phosphate (Na₂HPO₄•12H₂O) 27.g, citric acid (C₆H₈O₇•H₂O) 12 g, EDTA disodium salt 37 g, dissolved in water and diluted to 1,000 mL. 2. Sample purification ProElut™ PLS 150 mg / 6 mL (Cat#68004) Condition: 6 mL MeOH / 6 mL H₂O Load: 20 mL sample Wash 1: 6 mL H₂O Wash 2: 6 mL 10 % MeOH in H₂O Elute: 6 mL MeOH Reconstitute: Evaporate to near dry at 40 ºC, reconstitute to 1 mL with mobile phase 20 mL reservoir (Cat#4811) and adaptor (Cat#4803) is avilable for large volume sample
Conditions:	HPLC method Column: Spursil™ 5 µm C18, 150 x 4.6 mm (Cat#82001) Mobile Phase: A: 0.01 M Oxalic acid in H₂O, B: MeOH:MeCN = 1:1 Flow Rate: 1.0 mL/min Temperature: 30 ºC Detection: UV 365 nm Injection Volume: 20 µL
Author:	Dikma Technologies Inc.
Keyword:	Tetracyclines, Milk, Dairy Products, Spursil, SPE, PLS