Determination of Tetracyclines in Milk and Dairy Products

Method: SPE
Matrix: Milk
Application No.: 101743
Columns: ProElut PLS 150 mg / 6 mL 30/pk
Cat No.: 68004
Sample Pretreatment: 1. Sample preparation
Dilute milk sample (20 mL) or 2 g solid dairy product with 20 mL H2O, add 20 mL McIlvaine buffer*, vortex for 2 min, centrifuge at 4,000 rpm for 10 min, take 20 mL supernatant as the sample solution.
*McIlvaine buffer: Disodium hydrogen phosphate (Na2HPO4•12H2O) 27.g, citric acid (C6H8O7•H2O) 12 g, EDTA disodium salt 37 g, dissolved in water and diluted to 1,000 mL.
2. Sample purification
ProElut™ PLS 150 mg / 6 mL (Cat#68004)
Condition: 6 mL MeOH / 6 mL H2O
Load*: 20 mL sample
Wash 1: 6 mL H2O
Wash 2: 6 mL 10 % MeOH in H2O
Elute: 6 mL MeOH
Reconstitute: Evaporate to near dry at 40 ºC, reconstitute to 1 mL with mobile phase
*20 mL reservoir (Cat#4811) and adaptor (Cat#4803) is avilable for large volume sample
Conditions: HPLC method
Column: Spursil™ 5 µm C18, 150 x 4.6 mm (Cat#82001)
Mobile Phase: A: 0.01 M Oxalic acid in H2O, B: MeOH:MeCN = 1:1
Flow Rate: 1.0 mL/min
Temperature: 30 ºC
Detection: UV 365 nm
Injection Volume: 20 µL
Author: Dikma Technologies Inc.
Keyword: Tetracyclines, Milk, Dairy Products, Spursil, SPE, PLS