Determination of Phenols in Water

Method: SPE
Matrix: Water
Application No.: 101748
Columns: ProElut PLS 60 mg / 3 mL 50/pk
Cat No.: 68003
Sample Pretreatment: 1. Sample preparation
100 mL sample, adjust to pH 2 with H3PO4
2. Sample purification
ProElut™ PLS 60 mg / 3 mL (Cat#68003)
Condition: 3 mL MeOH:MTBE = 10:90 / 3 mL MeOH / 3 mL H2O
Load: 100 mL sample, flow rate ≤ 5 mL/min
Wash: 3 mL H2O
Elute: 3 mL MeOH:MTBE = 10:90
Reconstitute: Evaporate at 40 ºC by N2, reconstitute to 1 mL with MeCN:H2O (50:50, V / V) solution
Conditions: HPLC method
Column: Inspire™ 5 µm C18, 150 x 4.6 mm (Cat#81001)
Mobile Phase: A: 1% CH3COOH in H2O, B: 1% CH3COOH in MeCN
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detection: UV 280 nm
Injection Volume: 20 µL
Author: Dikma Technologies Inc.
Keyword: Phenols, SPE, PLS, Inspire