Determination of Phthalate Esters (PAEs) in Water

Method: SPE
Matrix: Water
Application No.: 101750
Columns: ProElut PLS Glass Cartridge, 200 mg / 6 mL 30/pk
Cat No.: 68012G
Sample Pretreatment: 1. Sample purification
ProElut™ PLS GLASS 200 mg / 6 mL (Cat#68012G)
Condition: 6 mL methyl tert-butyl ether / 6 mL MeOH / 6 mL H2O
Load: Up to 500 mL sample, flow rate ≤15 mL/min
Wash: 3 mL 5 % MeOH / H2O
Elute: 3 mL MeOH / 6 mL methyl tert -butyl ether
Reconstitute: Evaporate to near dry at 30 ºC, reconstitute to 1 mL with acetonitrile
Conditions: HPLC method
Column: Inspire™ 5 µm C18, 250 x 4.6 mm (Cat#81006)
Mobile Phase: A: H2O, B: MeCN
Flow Rate: 1.0 mL/min
Temperature: 30 ºC
Detection: UV 230 nm
Injection Volume: 20 µL
Author: Dikma Technologies Inc.
Keyword: Phthalate Esters, PAEs, Inspire, SPE, PLS