Determination of Tetracyclines in Serum

Method:	SPE
Matrix:	Serum
Application No.:	101752
Columns:	ProElut PLS 60 mg / 3 mL 50/pk
Cat No.:	68003
Sample Pretreatment:	1. Sample preparation 2 mL serum, add 40 µL H₃PO₄ 2. Sample purification ProElut™ PLS 60 mg / 3 mL (Cat#68003) Condition: 3 mL MeOH / 3 mL H₂O Load: 2 mL sample Wash: 3 mL 5 % MeOH in H₂O Elute: 3 mL MeOH Reconstitute: Evaporate to near dry at 30 ºC, reconstitute to 1 mL with mobile phase
Conditions:	HPLC method Column: Inspire™ 5 µm C18, 250 x 4.6 mm (Cat#81006) Mobile Phase: MeOH:MeCN:10 mM oxalic acid in H₂O = 15:15:70 Flow Rate: 1.0 mL/min Temperature: 30 ºC Detection: UV 365 nm Injection Volume: 20 µL
Author:	Dikma Technologies Inc.
Keyword:	Tetracyclines, Serum, Inspire, SPE, PLS