Determination of Migration of Bisphenol A (BPA) from Plastic Baby Bottles

Method: SPE
Matrix: Plastic
Application No.: 101755
Columns: ProElut PLS Glass Cartridge, 200 mg / 6 mL 30/pk
Cat No.: 68012G
Sample Pretreatment: 1. Sample purification
Wash bottles, dry completely. Add distilled water so that each 8 m2 of plastic contact area corresponds to 10 mL of simulant immersion. Sealed with aluminum foil, place in oven at 100 ºC for 1 h. Cool to room temperature, then transfer to glass bottles, and seal until detection.
2. Sample purification
ProElut™ PLS GLASS 200 mg / 6 mL (Cat#68012G)
Condition: 6 mL MeOH / 6 mL H2O
Load: Adding sample
Wash: 6 mL 5 % MeOH in H2O
Elute: 6 mL MeOH
Reconstitute: Evaporate to near dry at 40 ºC, reconstitute to 1 mL with mobile phase
Conditions: HPLC method
Column: Inspire™ 5 µm C18, 250 x 4.6 mm (Cat#81006)
Mobile Phase: MeCN:2% CH3COOH in H2O = 40:60
Flow Rate: 1.0 mL/min
Temperature: 30 ºC
Detection: FLD Ex: 227 nm, Em: 313 nm
Injection Volume: 20 µL
Author: Dikma Technologies Inc.
Keyword: Migration of Bisphenol A (BPA) from Plastic Baby Bottles, Bisphenol A, BPA, Inspire, SPE, PLS