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Caffeine Metabolites

Method: HPLC
Matrix: Medicine
Application No.: 101908
Columns: Spursil C18 5 μm 150 x 4.6 mm
Cat No.: 82001
Conditions: Column: Listed on chromatograms
Dimension: 150 x 4.6 mm
Mobile Phase: MeOH:10 mM K2HPO4 (pH 7) = 3:97
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detection: UV 254 nm
Author: Dikma Technologies Inc.
Keyword: Caffeine Metabolites, spursil, Uric acid, Xanthine, 7-Methylxanthine, 1-Methyluric acid, 3-Methylxanthine, 1,3-Dimethyluric acid, Theobromine, 1,7-Dimethylxanthine, Theophylline

 
The separation of nine caffeine metabolites, each a geostereoisomer of similar structure, demonstrates the resolving power for positional isomers. The Spursil™ C18 column completes this separation in less than 13 minutes, with remarkable selectivity.
   
   
Table: 1. Uric acid
2. Xanthine
3. 7-Methylxanthine
4. 1-Methyluric acid
5. 3-Methylxanthine
6. 1,3-Dimethyluric acid
7. Theobromine
8. 1,7-Dimethylxanthine
9. Theophylline