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LC/MS/MS Method for Determination of Antitussives

Method: HPLC-MS
Matrix: Medicine
Application No.: 101937
Componds: Butethamate citrate, Dextromethorphan hydrobromide, Carbetapentane citrate, Oxeladin citrate, Cloperastine, hydrochloride
Columns: Endeavorsil C18 1.8μm , 150x2.1mm
Cat No.: 87004
Sample Pretreatment: Samples: Stock solution of each antitussives was prepared in acetonitrile at a concentration of 1 mg/mL. Combined working solution was freshly obtained by dilution of stock solution with mobile phase.
Conditions: Mobile Phase:
A: 0.1% formic acid in 20M ammonium formate
B: 0.1% formic acid in 25/25/50(v/v/v) ethanol:methanol: acetonitrile
Gradient: 10%B hold 1.5min, 10 to 30%B in 2.5min, 30 to 40%B in 2min, 40 to 50%B in 4min, 50 to 70%B in 3min, hold 4min, 70 to 10%B in 0.5min, equilibrate at 10%B for 3.5 min
Scan mode: ESI+;
Flow Rate: 0.2mL/min;
Injection: 2µL;
Temperature: 25℃
Publisher: Dikma Technologies Inc.
Keyword: Antitussives, LC/MS/MS, Butethamate citrate, Dextromethorphan hydrobromide, Carbetapentane citrate, Oxeladin citrate, Cloperastine, hydrochloride
Abstract: These medicines have similar molecular structures, they can be well separated on DIKMA Endeavorsil C18 Columns(Cat#87004) within 15 mins, coupled with sensitive MS analysis.

 

No.

Compounds

Concentration (ng/mL)

Retention Time(min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Butethamate citrate

20

10.5

264.4→91.1

264.4→191.1

2

Dextromethorphan hydrobromide

20

10.7

272.4→215.2

272.4→147.1

3

Carbetapentane citrate

10

13.4

334.4→100.2

334.4→145.1

4

Oxeladin citrate

10

13.8

336.5→100.1

336.5→72.2

5

Cloperastine hydrochloride

10

14.1

330.3→201.1

330.3→165.2