Lab Glassware Division 中文(Chinese)

UHPLC/MS/MS Determination of Six Flavonoids

Method: HPLC-MS
Matrix: 0
Application No.: 101951
Componds: Catechin hydrate, Rutin trihydrate, Quercetin, Baicalein
Chrysin, Flavone
Columns: Endeavorsil C18 1.8 μm 100 x 2.1 mm
Cat No.: 87003
Sample Pretreatment: Samples: Baicalein was dissolved in mixed solution of acetonitrile and water (1:1 v/v). Flavone was prepared by acetonitrile. Other standard stock solutions were made by ethanol. Each standard stock solution was prepared at 1 mg/mL.
Conditions: Mobile Phase: A: 10 mM ammonium formate, pH 2.1
B:acetonitrile / methanol = 6 / 4
Gradient: hold 10% B for 1.5 min, 10% to 70% Bin2.5min, 70 to 90%B in 4 min, hold2 min, 90 to 10%B in 1 min, equilibrate at 10%B for 6 min
Scan mode: ESI+;
Flow Rate: 0.2 mL/min;
Injection: 2 µL;
Temperature: 25℃
Publisher: Dikma Technologies Inc.
Keyword: Flavonoids, Catechin hydrate, Rutin trihydrate, Quercetin, Baicalein Chrysin, Flavone, UHPLC/MS/MS
Abstract: These compounds were separated on a DIKMA Endeavorsil® C18 column with good resolution and peak shapes in a total run time of 10 minutes.

 

No.

Compound

Concentration (µg/mL)

Retention Time(min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Catechin hydrate

4

4.37

291.2→139.0

291.2→123.0

2

Rutin trihydrate

5

4.82

611.1→303.0

611.1→465.1

3

Quercetin

3

5.70

303.0→229.0

303.0→153.2

4

Baicalein

0.1

6.35

271.1→ 123.0

271.1→95.0

5

Chrysin

0.2

7.17

255.0→153.0

255.0→69.0

6

Flavone

0.02

7.48

223.1→121.0

223.1→77.1