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Cefonicid Sodium

Method: HPLC
Matrix: Medicine
Application No.: 101961
Componds: Cefonicid Sodium, Desacetyl Cefonicid
Columns: Diamonsil® Plus C18 5μm 150 x 4.6 mm
Cat No.: 99401
Sample Pretreatment: Resolution solution: Dissolve suitable quantities of Cefonicid Sodium in mobile phase to obtain a solution having a known concentration of 0.2 mg per mL. Heat on a steam bath for 30 minutes, and cool. This resolution solution contains a mixture of cefonicid and desacetyl cefonicid.
Conditions: Mobile phase:
A mixture of water, methanol, and 0.2 M monobasic ammonium phosphate (31.6: 6.5: 1.9)
Detector: UV 254 nm
Flow rate: 2.0 mL/min
Injection volume: 3 µL
Column temp.: 30 ℃
Publisher: Dikma Technologies Inc.
Keyword: Cefonicid Sodium, Desacetyl Cefonicid, HPLC, Diamonsil Plus C18
Abstract: Cefonicid Sodium is the sodium salt of cefonicid, a semisynthetic second-generation cephalosporin with antibacterial activity. The experimental conditions follow guidelines from the USP43-NF38 monograph method.

 

Peaks

Compound

Retention Time

(min)

Resolution

Tailing Factor

Theoretical Plates

1

Cefonicid Sodium

2.713

-

1.09

33233

2

Desacetyl Cefonicid

3.105

2.50

1.03

40300