Analysis of 14 Nonsteroidal Anti-inflammatory Drugs by A HPLC Method

Method:	HPLC
Matrix:	Medicine
Application No.:	101960
Componds:	Acetaminophen, 4-Aminosalicylic acid, Salicylamide, Acetylsalicylic acid, Phenacetin, Piroxicam, Ketorolac, Sulindac, Ketoprofen, Fenoprofen calcium, Flurbiprofen, Diclofenac sodium, Indomethacin, Ibuprofen
Columns:	Diamonsil® Plus C18 5μm 250 x 4.6 mm
Cat No.:	99403
Sample Pretreatment:	Standards: Individual stock solution was prepared in acetonitrile/water (4/1 v/v) at a concentration of 1.0 mg/mL. A working solution of the mixture of all 14 drugs was obtained by dilution of the stock solution with mobile phase.
Conditions:	Mobile Phase: A: 30mM Potassium Phosphate Monobasic (pH 3.1) B: Acetonitrile / Methanol = 4 / 6 (v/v) Gradient: hold 10% B for 0.5 min, 10 to 60% B in 11.5 min, 60 to 70% B in 11 min, hold 70% B for 5 min, 70 to 10% B in 1 min, equilibrate at 10% B for 6 min. Flow Rate: 1.0 mL/min Injection: 10 µL Temperature: 30 ℃ Wavelength: 230 nm
Publisher:	Dikma Technologies Inc.
Keyword:	Acetaminophen, 4-Aminosalicylic acid, Salicylamide, Acetylsalicylic acid, Phenacetin, Piroxicam, Ketorolac, Sulindac, Ketoprofen, Fenoprofen calcium, Flurbiprofen, Diclofenac sodium, Indomethacin, Ibuprofen, HPLC, Diamonsil Plus C18
Abstract:	Analysis was carried out on a DIKMA Diamonsil Plus C18 column with gradient elution time within 27 min. This method could be used routinely for drug QC inspection or further studies.