Determination of Fungicides in Fruit Juice

Method:	SPE
Matrix:	Juice
Application No.:	101736
Columns:	ProElut PXC 60 mg / 3 mL 50/pk
Cat No.:	68203
Sample Pretreatment:	1. Sample preparation 1.1 Juice drinks and pure fruit juice Start with 10 mL sample, adjust to pH 10 - 11 with 0.1 M NaOH, add 15 mL ethyl acetate, shake 1 min, centrifuge 1 min at 4,000 rpm, collect ethyl acetate layer. Repeat 15 mL ethyl acetate extraction, and combine organic phases, vacuum distillation at 30 ºC to near dry. Dissolve residue with 0.1 M HCl (6 mL) twice. 1.2 Fruit juice concentrate Start with 2 mL sample mixed with 8 mL H₂O, adjust to pH 10 - 11 with 0.1 M NaOH, and then follow the above steps. 2. Sample purification ProElut™ PXC 60 mg / 3 mL (Cat#68203) Condition: 3 mL MeOH / 3 mL H₂O Load: Adding sample Wash: 3 mL H₂O / 3 mL MeOH Elute: 3 mL MeOH (5 % NH₄OH) Reconstitute: Evaporate to near dry at 30 ºC, reconstitute to 1 mL with mobile phase
Conditions:	HPLC method Column: Inspire™ 5 µm C18, 250 x 4.6 mm (Cat#81006) Mobile Phase: Phosphate buffer:MeCN = 75:25 Flow Rate: 1.0 mL/min Temperature: 30 ºC Detection: UV 288 nm Injection Volume: 20 µL Phosphate buffer: 1.38 g sodium dihydrogen phosphate, 1.41 g disodium hydrogen phosphate, dissolve in 1,000 mL water
Author:	Dikma Technologies Inc.
Keyword:	Fungicides, Fruit Juice, Inspire, SPE, PXC