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Simultaneous Determination of 8 Penicillins Using UHPLC/MS/MS Method

Method: HPLC-MS
Matrix: Medicine
Application No.: 101932
Componds: Ampicillin sodium, Methicillin sodium, Piperacillin sodium, Penicillin G sodium, Penicillin V potassium, Oxacillin sodium, Nafcillin sodium, Dicloxacillin sodium
Columns: Endeavorsil C18 1.8μm , 150x2.1mm
Cat No.: 87004
Sample Pretreatment: Standards: Individual stock solutions of most of the drugs were prepared in a mixed solution of acetonitrile and water (9:1 v/v) at a concentration of 1 mg/mL. A working solution of the mixture of all 8 drugs was obtained by dilution of the stock solution with mobile phase.
Conditions: Mobile Phase:
A: 10 mM ammonium formate, pH 2.0
B: methanol:acetonitrile = 4:6
Gradient: hold 30% B for 1.5 min, 30 to 80% B in 11.5 min, hold 80% B for 1.5 min, 80 to 30% B in 0.5 min, equilibrate at 30% B for 5 min.
Flow Rate: 0.2 mL/min;
Injection: 2 µL;
Temperature: 25 ℃
Source: ESI; Ionization Mode: Positive
Publisher: Dikma Technologies Inc.
Keyword: Ampicillin sodium, Methicillin sodium, Piperacillin sodium, Penicillin G sodium, Penicillin V potassium, Oxacillin sodium, Nafcillin sodium, Dicloxacillin sodium, Penicillins, UHPLC/MS/MS
Abstract: In this method, a DIKMA Endeavorsil® 1.8 µm C18 column is used for successful separation of the 8 compounds within 13 min.

 

No.

Compounds

Concentration (µg/mL)

Retention Time (min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Ampicillin sodium

1.0

2.65

350.1→106.0

350.1→192.0

2

Methicillin sodium

0.2

8.31

381.1→165.1

381.1→222.1

3

Piperacillin sodium

0.5

8.55

518.1→143.0

518.1→160.0

4

Penicillin G sodium

0.3

9.32

335.1→160.0

335.1→176.0

5

Penicillin V potassium

0.5

10.49

351.1→160.0

351.1→114.0

6

Oxacillin sodium

0.5

10.88

402.0→243.0

402.0→160.0

7

Nafcillin sodium

0.1

12.11

415.1→199.1

415.1→171.1

8

Dicloxacillin  sodium

1.0

12.72

470.0→160.1

470.0→311.0