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Fast LC/MS/MS Analysis of Antihistamines Using Endeavorsil C18 Columns

Method: HPLC-MS
Matrix: Medicine
Application No.: 101940
Componds: Pheniramine, Doxylamine, Pyrilamine, Chlorpheniramine, Triprolidine, Carbinoxamine, Desloratidine, Astemizole, Diphenhydramine, Promethazine,
Azelastine, Hydroxyzine, Levocetirizine, Terfenadine, Meclizine
Columns: Endeavorsil C18 1.8 μm 50 x 2.1 mm
Cat No.: 87002
Sample Pretreatment: Samples: A standard mixture of 15 analyteswas prepared with each standard stock solution of 1mg/mL.
Conditions: Mobile Phase: A: 0.1% trifluoroacetic acid in water
B:0.02% trifluoroacetic acid in 75:25(v/v) acetonitrile:methanol
Gradient: 10%B hold 0.5min, 10 to 25%B in 7.5min, 25 to 90%B in 4min, hold 1min, 90 to 10%B in 0.1min, equilibrate at 10%B for 3.9 min
Scan mode: ESI+;
Flow Rate: 0.2mL/min;
Injection: 10µL;
Temperature: 25℃
Publisher: Dikma Technologies Inc.
Keyword: LC/MS/MS, Antihistamines, Endeavorsil C18, Pheniramine, Doxylamine, Pyrilamine, Chlorpheniramine, Triprolidine, Carbinoxamine, Desloratidine, Astemizole, Diphenhydramine, Promethazine, Azelastine, Hydroxyzine, Levocetirizine, Terfenadine, Meclizine
Abstract: In this report, 15 antihistamines can be rapidly detected on a 50mm Endeavorsil C18 Column

 

No.

Compounds

Retention Time(min)

Precursor Ion

Product Ion 1

Product Ion 2

1

Pheniramine

5.38

241.3

196.0

167.1

2

Doxylamine

5.54

271.2

167.1

182.0

3

Pyrilamine

7.85

286.2

121.0

241.2

4

Chlorpheniramine

9.61

275.2

230.0

167.1

5

Triprolidine

9.78

279.2

208.0

193.0

6

Carbinoxamine

9.93

291.2

167.1

202.2

7

Desloratidine

10.56

311.2

294.0

259.2

8

Astemizole

11.38

459.3

135.1

218.1

9

Diphenhydramine

11.66

256.3

167.1

152.2

10

Promethazine

11.92

285.2

240.0

198.0

11

Azelastine

12.00

382.4

112.1

159.0

12

Hydroxyzine

12.01

375.2

201.0

166.1

13

Levocetirizine

12.01

389.3

201.0

166.1

14

Terfenadine

12.66

472.4

454.2

436.3

15

Meclizine

12.73

391.3

201.0

166.0