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Analysis of H2 Antagonists Using an HPLC method

Method: HPLC
Matrix: Medicine
Application No.: 101943
Componds: Nizatidine, Famotidine, Cimetidine, Ranitidine hydrochloride
Columns: Spursil C18 3 μm 250 x 4.6 mm
Cat No.: 82020
Sample Pretreatment: Standards: Each single stock solution was prepared at a concentration of 1mg/mL using amixed solution of methanol and acetonitrile (1:5 v/v).
Conditions: Mobile Phase:
A: 40mMPotassium phosphate monobasicbuffer (pH2.8)
B: Acetonitrile/methanol=60:40
Gradient: 5%B hold 1 min, 5 to 12%B in 14min, hold 2min, 12 to 5%B in 1min,equilibrate at 5%B for 7min
Flow Rate: 1.0 mL/min;
Injection: 10µL;
Temperature: 30℃;
Wavelength: 230nm
Publisher: Dikma Technologies Inc.
Keyword: Nizatidine, Famotidine, Cimetidine, Ranitidine hydrochloride, HPLC, H2 Antagonists
Abstract: These four H2 antagonistscould be well separated on a DIKMA SpursilC18 column with good peak shapeand efficient elution out of the column within 16 minutes.

 

No.

Compounds

Concentration (mg/mL)

Retention Time(min)

Resolution

Asymmetry

1

Nizatidine

0.01

11.6

-

1.11

2

Famotidine

0.01

14.4

11.98

1.10

3

Cimetidine

0.01

14.9

2.04

1.10

4

Ranitidine hydrochloride

0.01

15.6

2.71

1.09