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Analysis of AZBT by HPLC method

Method: HPLC
Matrix: Medicine
Application No.: 101954
Componds: 5-(4’-(AZIDOMETHYL)- [1,1’-BIPHENYL] -2-YL)-1H-TETRAZOLE
CAS: 152708-24-2
Columns: Diamonsil® Plus C18 5μm 250 x 4.6 mm
Cat No.: 99403
Sample Pretreatment: Stock solution: 1 mg/mL of AZBT in acetonitrile
Working solution: 20 μg/mL of AZBT in acetonitrile/water (v/v, 50/50)
Conditions: Mobile phase:
A: 0.05% trifluoroacetic acid in water
B: 0.04% trifluoroacetic acid in acetonitrile/water (90/10, v/v)
Gradient:
Time/min A/% B/%
0 70 30
1 70 30
13 10 90
18 10 90
19 70 30
25 70 30
Detection: UV 220nm
Flow rate: 1.0 mL/min
Injection volume: 10μL
Column temp.: 25℃
Publisher: Dikma Technologies Inc.
Keyword: AZBT, HPLC, Diamonsil Plus C18
Abstract: 5-(4’-(AZIDOMETHYL)- [1,1’-BIPHENYL] -2-YL)-1H-TETRAZOLE also called as AZBT, is an azide impurity that found in sartans drugs.