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Analysis of AZBT by LC/MS/MS method

Method: HPLC-MS
Matrix: Medicine
Application No.: 101955
Componds: 5-(4’-(AZIDOMETHYL)- [1,1’-BIPHENYL] -2-YL)-1H-TETRAZOLE
CAS: 152708-24-2
Columns: Navigatorsil 2.7 μm C18, 100 x 2.1 mm
Cat No.: 88003
Sample Pretreatment: Stock solution: 1 mg/mL of AZBT in acetonitrile
Working solution: 0.02 μg/mL of AZBT in acetonitrile/water (v/v, 10/90)
Conditions: Mobile phase:
A: 0.1% formic acid in water
B: 0.1% formic acid in acetonitrile
Gradient:
Time/min A/% B/%
0 70 30
3 70 30
7 10 90
11 10 90
11.1 70 30
15 70 30
Flow rate: 0.3 mL/min
Injection volume: 2μL
Column temp.: 25℃
Ionization mode: ESI+
Acquisition mode: MRM
Publisher: Dikma Technologies Inc.
Keyword: AZBT, LC/MS/MS, Navigatorsil C18
Abstract: 5-(4’-(AZIDOMETHYL)- [1,1’-BIPHENYL] -2-YL)-1H-TETRAZOLE also called as AZBT, is an azide impurity that found in sartans drugs.