Determination of Four Serotonin and Norepinephrine Reuptake Inhibitors by a HPLC Method

Method:	HPLC
Matrix:	Medicine
Application No.:	101964
Componds:	Milnacipran hydrochloride, Venlafaxine hydrochloride, Atomoxetine hydrochloride. Duloxetine hydrochloride
Columns:	Diamonsil® Plus C18 5μm 150 x 4.6 mm
Cat No.:	99401
Sample Pretreatment:	Standards: Individual stock solutions were prepared in acetonitrile at a concentration of 1 mg/mL. A working solution of the mixture of four drugs was obtained by dilution of the stock solution with mobile phase A
Conditions:	Mobile Phase: A: 40mM Ammonium Acetate (pH5.5) B: methanol:acetonitrile = 6:4 A: B = 50:50 Flow Rate: 1 mL/min Injection: 5 µL Temperature: 30 ℃ Wavelength: 220 nm
Publisher:	Dikma Technologies Inc.
Keyword:	Milnacipran hydrochloride, Venlafaxine hydrochloride, Atomoxetine hydrochloride. Duloxetine hydrochloride, HPLC, Diamonsil Plus C18
Abstract:	A HPLC method was developed using the DIKMA Diamonsil Plus C18 column. Four SNRIs were well separated within 8.5 min with simple isocratic elution procedure.