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Equilin

Method: HPLC
Matrix: Medicine
Application No.: 101966
Componds: Phenol, Equilin
Columns: Inspire C18 5 μm 250 x 4.6 mm
Cat No.: 81006
Sample Pretreatment: System suitability solution: dissolve suitable quantities of Equilin and phenol in acetonitrile to obtain a solution containing about 0.2 and 0.035 mg per mL, respectively.
Conditions: Mobile phase: Acetonitrile and water (45:55)
Detector: UV 280 nm
Flow rate: 1.0 mL/min
Injection volume: 10 µL
Column temp.: 30 ℃
Publisher: Dikma Technologies Inc.
Keyword: Phenol, Equilin, HPLC, Inspire C18
Abstract: Equilin is a naturally occurring estrogen sex hormone found in horses as well as a medication. The experimental conditions follow guidelines from the USP43-NF38 monograph method.

 

Peaks

Compound

Retention Time

(min)

Resolution

Tailing Factor

1

Phenol

4.946

-

1.05

2

Equilin

13.644

31.19

1.02