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Estrone

Method: HPLC
Matrix: Medicine
Application No.: 101967
Componds: Estrone
Columns: Diamonsil® Plus C18 5μm 150 x 4.6 mm
Cat No.: 99401
Sample Pretreatment: System suitability solution: Transfer about 2 mg of Estrone, accurately weighed, to a 10-mL volumetric flask, add methanol to volume, and mix. If necessary, sonicate to aid solution. Transfer 1.0 mL of this solution and dilute with Mobile phase to 5 mL, and mix.
Conditions: Mobile phase: Acetonitrile and 0.05M monobasic potassium phosphate (1:1)
Detector: UV 280 nm
Flow rate: 1.0 mL/min
Injection volume: 10 µL
Column temp.: 30 ℃
Publisher: Dikma Technologies Inc.
Keyword: Estrone, HPLC, Diamonsil Plus C18
Abstract: Estrone is a steroid, a weak estrogen, and a minor female sex hormone. The experimental conditions follow guidelines from the USP43-NF38 monograph method.

 

Compound

Retention Time

(min)

Theoretical Plates

Tailing Factor

Estrone

5.066

81227

1.07