Lab Glassware Division 中文(Chinese)

UHPLC/MS/MS Method for Proxalutamide Determination

Method: HPLC-MS
Matrix: Medicine
Application No.: 101976
Componds: Proxalutamide
Columns: Navigatorsil 2.7 μm C18, 100 x 2.1 mm
Cat No.: 88003
Sample Pretreatment: Samples: Working solution of 0.02 µg/mL was obtained by dilution of the stock solution with acetonitrile/water (1/9, v/v). Standard solutions should be stored at -20 ℃.
Conditions: Mobile Phase:
A: water
B: acetonitrile
Gradient: hold 40% B for 0.5 min, 40% to 80% B in 4.5 min, hold 1 min, 80 to 40% B in 0.1 min, equilibrate at 40% B for 5.9 min
Scan mode: ESI+
Flow Rate: 0.3 mL/min
Injection: 2 µL
Temperature: 25 ℃
Publisher: Dikma Technologies Inc.
Keyword: Proxalutamide, UHPLC/MS/MS, Navigatorsil C18
Abstract: In this application is within 4 mins, and mobile phases used only are water and acetonitrile.

 

Compounds

Concentration (µg/mL)

Retention Time(min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

Proxalutamide

0.02

3.86

518.0 → 96.1

518.0 → 435.0